FIGURE 3.

MKP-1 is required for antigen-specific T cell responses in vivo. WT and KO mice (n = 5) were immunized with OVA in CFA. On day 8, splenocytes were isolated and stimulated with peptide as indicated. A, IL-2 production and cell proliferation were measured after 1 and 3 days of stimulation, respectively. B, cytokine production was determined by ELISA after 3 days of stimulation. C, anti-OVA antibodies of different isotypes in sera from immunized mice were measured by ELISA. The data are representative of two independent experiments with similar results.