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. 2009 Sep 9;284(45):30862–30870. doi: 10.1074/jbc.M109.046201

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — SDS-polyacrylamide gel of 58-HCS purification fractions. A, lane 1, low range molecular weight markers (Bio-Rad). Lane 2, crude extract after induction. Lane 3, sample obtained from the first Ni²⁺-Sepharose column. Lane 4, sample after SUMO protease 1 cleavage. Lane 5, flow-through from the second Ni²⁺-Sepharose column. Lane 6, material obtained after Toyopearl anion exchange column. B, 12% SDS-polyacrylamide gel of the pure proteins. Lane 1, low range molecular weight markers. Lane 2, FL-HCS; lane 3, 58-HCS.