TABLE 2.

Kinetic parameters for ATP dependence of bio-5′-AMP synthesis

The kinetic parameters were determined by mixing a complex of HCS-biotin with ATP as described under “Experimental Procedures.”

HCS form	k1	Km	kcat	kcat/Km
	×103m−1s−1	μm	s−1	×103m−1s−1
58	4.9 ± 0.7a	50 ± 30a	0.12 ± 0.02a	2 ± 1
			(0.39 ± 0.06)b	8 ± 5
FL	6 ± 1c	50 ± 20c	0.11 ± 0.02c	2 ± 1
			(0.36 ± 0.06)b	7 ± 3

^a The errors correspond to the standard deviation for two independent experiments.

^b The calculated k_cat values were obtained at 37 °C assuming that the rate doubles every 10 °C.

^c The errors correspond to the standard deviation for three independent experiments.