FIGURE 4.

Transgenic BCL2 and MCL1 inhibit FAS- and GC-induced apoptosis in G₀/G₁-arrested CEM/p16 cells. A, ectopic expression of BCL2 and MCL1 in CEM-1D2/p16-bcl2, CEM-6E2/p16-bcl2, CEM-1D2/p16-mcl1, and CEM-6E2/p16-mcl1 cells was determined by immunoblot analyses. Equal protein loading was confirmed by α-tubulin (Tub) staining. B and C, CEM/Ctr, CEM/p16-ctr, CEM/p16-bcl, and CEM/p16-mcl1 cells were incubated with 250 ng/ml Dox for 24 h and then treated with 0.1 μg/ml anti-FAS antibody for 4 h or with 10 nm Dex for 40 h. Apoptosis induction was measured by PI-FACS analysis. Shown is the mean ± S.E. of four independent experiments. Statistical analysis was performed using unpaired t test. **, p < 0.01; ***, p < 0.0001 for Bcl2- or Mcl1-transgenic CEM/p16-cells compared with CEM/p16 controls (ctr).