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Kinetic constants for wild-type and mutant human PNP oxidase
Enzymatic activity of wild-type, R229W, and R229Q enzymes was measured by following the formation of aldimine complex of PLP with Tris at 414 nm. Km and kcat values are average of at least 3 measurements.
| Enzyme | Km for PNP | kcat |
|---|---|---|
| μm | s−1 | |
| Wild type | 2.4 ± 0.2 | 0.18 ± 0.03 |
| R229W | 461 ± 27 | 0.04 ± 0.01 |
| R229Q | 310 ± 20 | 0.05 ± 0.01 |
