FIGURE 3.

Stabilized HM and QM mutants gave higher fluorescence (levels of folded p53) than wild-type p53 over a time course. EGFP-tagged transcriptionally active p53 mutants were transfected into H1299 cells, and the average fluorescence intensity was measured by FACS scan at different time points from 12 to 48 h after transfection. Higher thermodynamic stability led to higher accumulation over time. The QM-V143A control, which was similar to WT in terms of thermodynamic stability, had similar levels of folded protein to WT.