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. 2009 Aug 21;284(45):30974–30980. doi: 10.1074/jbc.M109.033183

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Higher stability of p53 leads to higher fluorescence (levels of folded p53) in both transcriptionally active and inactive (QS1/2) p53 mutants. a, EGFP-tagged p53 mutants were transfected into H1299 cells and the average fluorescence intensity was measured 48 h after transfection using a FACscan. Levels of folded p53 were correlated with intrinsic thermodynamic stability. Transcriptionally active mutants had lower levels of folded protein than transcriptionally inactive ones with the QS1/2 scaffold. b, Western blot showing the total fraction of p53 protein 48 h after transfection. The total fraction of p53 correlates well with thermodynamic stability of the transcriptionally inactive QS1/2 mutants.