FIGURE 5.

ADAM10 is essential for endogenous Notch1 Val¹⁷¹¹ cleavage. A, Adam17-deficient cells are transduced with Jagged1 ligand and used in co-culture experiments to stimulate wild type (WT) and Adam17-deficient cells that express endogenous Notch1 receptor. Immunoblotting for Notch1, Val¹⁷¹¹, and Val¹⁷⁴⁴ shows no defect is observed in Notch1 cleavage in the absence of ADAM17. ko, knock-out. B, in contrast in cells lacking ADAM10-expressing endogenous Notch1 receptor, S2 and S3 cleavages are severely impaired compared with wild type cells. Jagged1 and TACE/ADAM17 expression is shown. Asterisk indicates nonspecific reaction of TACE antibody.