Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Equilibrium titrations of E1 ↔ E2(Rb) at varying pH. A, wild type; B, D369N. The data have been normalized as described under “Experimental Procedures.” Solid lines represent curves fitted to these data and provide the parameters in Table 4. At pH values lower than 7.0, a linear drift was subtracted from the fluorescence signal changes.
