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Equilibrium titrations of the E1 ↔ E2(2Rb) conformational change
Parameters were obtained by fitting the normalized data in Fig. 4 to the Hill equation. The K+ titrations for the wild-type are derived from a separate experiment.
| K0.5Rb(K) | nH | |
|---|---|---|
| mm ± S.E. | ||
| Wild type, Rb+ | 5.60 ± 0.09 | 1.8 ± 0.04 |
| Wild type, K+ | 3.90 ± 0.04 | 1.7 ± 0.08 |
| D369N, Rb+ | 0.90 ± 0.02 | 2.1 ± 0.1 |
| D369A, Rb+ | 1.70 ± 0.07 | 1.98 ± 0.14 |
| D369E, Rb+ | 6.33 ± 1.0 | 1.63 ± 0.17 |
| T212A, Rb+ | 6.6 ± 0.08 | 2.3 ± 0.05 |
