TABLE 5.

Rates of E₁ → E₂·P and E₁ → E₂·vanadate conformational transitions

Rate constants obtained by double exponential fits of the data in Fig. 11.

	Wild type	D369N	D369A
	s−1 ± S.E.	s−1 ± S.E.	s−1 ± S.E.
E1 → E2·P	k1, 0.026 ± 0.001	k1, 0.67 ± 0.09	k1, 0.89 ± 0.05
	k2, 0.004 ± 0.0001	k2, 0.036 ± 0.002	k2, 0.038 ± 0.001
E1 → E2·vanadate	k1, 0.086 ± 0.001	k1, 12.18 ± 0.37	k1, 0.97 ± 0.18
	k2, 0.011 ± 0.0001	k2, 0.1 ± 0.006	k2, 0.17 ± 0.017