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. 2009 Sep 4;284(45):31085–31096. doi: 10.1074/jbc.M109.035576

FIGURE 5.

FIGURE 5.

hnRNP H2 binds to CUGBP1 in an RNA-independent manner. CUGBP1 (A) and hnRNP H (B) were coimmunoprecipitated from MCF-7 whole cell extracts using antibodies against hnRNP H and CUGBP1, respectively. The no antibody, IgG, and hemagglutinin (HA) coimmunoprecipitations serve as controls for nonspecific binding. CUGBP1 (C) and hnRNP H (D) were coimmunoprecipitated from a mixture of recombinant hnRNP H2 and recombinant CUGBP1 using antibodies against hnRNP H and CUGBP1, respectively. The no antibody and IgG coimmunoprecipitations serve as controls for nonspecific binding.