FIGURE 3.

Effect of calumenin knockdown on expression of Ca²⁺-cycling proteins in HL-1 cells. Western blot analysis for Ca²⁺-cycling proteins in HL-1 cells transfected with siRNA-control or siRNA-calumenin oligonucleotides. A, cells transfected with oligonucleotides were harvested 72 h after transfection, and cell lysates was prepared as described under “Experimental Procedures.” After immunoblotting, the blots were probed with RyR2, phospho-RyR2, SERCA2, Na⁺-Ca²⁺ exchanger, CSQ, dihydropyridine receptor, PLN, and Ser¹⁶ phospho-PLN antibodies. B, band intensities of proteins were normalized to the siRNA-control Western bands. Data represent means ± S.E., n = 3–4.