FIGURE 2.

hNaa50p/hSan acetylation of peptides with the N termini Met-Leu or Met-Asp. Purified GST-hNaa50p was incubated with selected oligopeptides and [1-¹⁴C]acetyl-CoA for 2 h at 37 °C. After incubation, the oligopeptides were isolated with SP-Sepharose beads, washed three times with 0.5 m acetic acid, and analyzed with scintillation counting. Activity detected in the negative control (dH₂O) was subtracted. The NAT complexes that are expected to perform the acetylation in vivo are given. Error bars (S.D.) are based on three independent experiments. For details see “Experimental Procedures.”