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. 2009 Sep 10;284(45):31130–31141. doi: 10.1074/jbc.M109.053751

FIGURE 2.

FIGURE 2.

Homodimerization of atToc33-R130A and atToc33-F67A. A, increasing concentrations of immobilized atToc33G-His6, atToc33G-R130A-His6, or atToc33G-F67A-His6 were incubated with full-length in vitro translated [35S]atToc33, [35S]atToc33-R130A, or [35S]atToc33-F67A, respectively. 10% of the in vitro translation (IVT) products added to each reaction is indicated (lanes 1 and 7). The graph presents quantitation of bound proteins from triplicate experiments corresponding to the representative data shown in the upper panels. B, increasing concentrations of immobilized CRABP-His10 were incubated with full-length in vitro translated [35S]atToc33, [35S]atToc33-R130A, or [35S]atToc33-F67A as a control for nonspecific binding. Bound proteins were analyzed by SDS-PAGE and quantified using phosphorimaging. The error bars in both panels correspond to S.D.