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. 2009 Sep 10;284(45):31130–31141. doi: 10.1074/jbc.M109.053751

FIGURE 5.

FIGURE 5.

Genotypic and phenotypic analysis of transgenic plants. A, the genotypes of WT and ppi1 plants transformed with atToc33.Tev.His (WT:ppi1), atToc33-R130A.Tev.His (R130A:ppi1), and atToc33-F67A.Tev.His (F67A:ppi1) were confirmed by PCR analysis of genomic DNA from plants with primer sets specific for the transgene (see “Experimental Procedures”). B, immunoblot analysis of levels of protein expression in isolated chloroplasts from 10-day-old plants grown on agar plates. Serial dilutions of chloroplast samples were immunoblotted with the affinity-purified antibodies as indicated. C, detergent-solubilized chloroplasts from WT:ppi1, R130A:ppi1, and F67A:ppi1 plants were subjected to immunoprecipitation with anti-atToc159 serum. The total fractions from the immunoprecipitates were resolved by SDS-PAGE and immunoblotted with affinity-purified antibody as indicated on each panel. D, visual phenotype of atToc33 transgenic plants from fully matured 30-day-old plants grown in soil. E, change in chlorophyll content of ppi1 transformants during development. The data in the graph represent the mean of three independent experiments at 4, 7, 10, and 22 days after germination. The error bars correspond to S.D.