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Expression of PTOX in E. coli. The immunoblot was decorated with polyclonal antibodies directed against PTOX. 250 μl of culture was taken as a sample at given times after induction by isopropyl-1-thio-β-d-galactopyranoside (0–4 h and overnight, o.n.), centrifuged, and the pellet was resuspended in 50 μl of SDS loading buffer. 10 μl were loaded.
