TABLE 2.
ROS production in control and PTOX-containing E. coli membranes
Membranes (0.4 mg protein ml−1) were incubated for 5 min with 5 mm succinate and, when indicated, 0.2 mm decyl-plastoquinone in the presence of the spin trap 4-POBN (50 mm)/ethanol. The double integral of the total signal obtained with control membranes in the presence of decyl-plastoquinone was set to unity. Five spectra were used to calculate the mean and the standard deviation.
| EPR signal size |
||
|---|---|---|
| Control membranes | PTOX-containing membranes | |
| No decyl-PQ | Not detectable | 0.6 ± 0.05 |
| +decyl-PQ | 1.0 ± 0.1 | 1.6 ± 0.1 |