FIGURE 2.
Pulldown affinity capture using His6-Hsp90 (bait) and AS (prey) at different stages of amyloidogenesis (M, marker; 1, background; 2, AS control; 3, after 0 h; 4, after 10 h; 5, after 25 h; 6, after 40 h; 7, after 0 h in the presence of 5 mm MgATP; 8, after 0 h in the presence of 5 mm MgATP+ 0.5 mm 17-AAG). The bands around 21 and 31 kDa correspond to partial degradation products of Hsp90. Fibrillogenesis was performed as described under “Experimental Procedures” (ThioT measurements), except that the AS solution was 200 μm and agitation was 1200 rpm.