TABLE 2.

Reductive half-reaction of recombinant GOX with glycolate as a substrate between pH 5.0 and 10.0

Flavin reduction was carried out in a stopped-flow spectrophotometer at 30 °C. Pre-steady-state kinetic parameters were determined by fitting the data acquired upon mixing anaerobically the enzyme with glycolate to Equation 7.

pH	k3	Kd(app)
	s−1	mm
5.0	26.8 ± 0.2	0.66 ± 0.02
6.0	45.9 ± 0.3	0.41 ± 0.01
7.0	52.0 ± 0.4	0.32 ± 0.01
8.0	43.8 ± 0.2	0.39 ± 0.01
9.0	39.9 ± 0.2	1.64 ± 0.03
10.0	34.6 ± 0.5	9.35 ± 0.38