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. 2009 Sep 9;284(45):31223–31235. doi: 10.1074/jbc.M109.041665

FIGURE 6.

FIGURE 6.

In vivo dexamethasone inhibits ATM accumulation. A, adipocyte size areas were calculated from paraffin-embedded epididymal fat tissue sections. Data are presented as the percentage of cells counted falling within the indicated ranges of sizes for mice either fed an HFD or fed an HFD and treated with dexamethasone (HFD + Dex). B, FACS sorting was performed on epididymal fat stromal vascular cells sorted for F4/80, CD11b, and CD11c. Data represent relative cell numbers of the reported macrophage subtypes for HFD versus HFD + Dex mice. C, dot plot representation of CD11b versus CD11c relative expression for FACS data obtained from F4/80 gated macrophages. D, MAC2 immunostaining on paraffin-embedded epididymal fat tissue sections from HFD versus HFD + Dex mice. E, QPCR analysis of CD68, RANTES, CCR2, and CCR5 expression on epididymal fat from normal chow diet, HFD, and HFD + Dex mice. Bars, average values ± S.D. of duplicates from at least three independent experiments. A–D above the bars show statistical groups (p < 0.05). NCD, normal chow diet; A.U., arbitrary units.