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. 2009 Sep 11;284(45):31260–31269. doi: 10.1074/jbc.M109.004382

FIGURE 1.

FIGURE 1.

22L prion infection of N2a cells induces increased transcription of genes regulated by Srebp2. Differential gene expression of genes involved in cholesterol synthesis is shown. A, up-regulation of Srebf2 (sterol regulatory element-binding factor 2), Mvk (mevalonate kinase), Fdft1 (farnesyl-diphosphate farnesyltransferase 1), Cyp51 (cytochrome P450, family 51), Ldlr (low density lipoprotein receptor), and Sc4mol (C4-sterol methyl oxidase) was determined by real time PCR in the 22L-infected clone 5 (passage 18) compared with mock-infected clone 5 (passage 18). All results were normalized to the RPII (RNA polymerase 2) expression levels. For triplicate experiments, the standard deviation is shown for each gene. The y axis denotes the comparative gene expression levels. B, overview of important genes in the cholesterol pathway activated by Srbp2 (17). Genes in boldface were found up-regulated in prion-infected N2a cells compared with mock-infected N2a cells either in the chip experiment (underlined) or as detected by real time PCR (marked with asterisks). Srebp2 is the limiting transcription factor of cholesterol synthesis. HMG-CoA, hydroxymethylglutaryl-CoA; FPP, farnesyl diphosphate; IPP, isopentenyl diphosphate; GPP, geranyl diphosphate; DHCR, 7-dehydrocholesterol reductase.