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. 2009 Aug 27;284(45):31303–31314. doi: 10.1074/jbc.M109.028910

FIGURE 1.

FIGURE 1.

ABCG1 is targeted for proteasomal degradation by 12SHETE. A–D, C57BL6/J bone marrow-derived macrophages were treated with vehicle control (CTR) or the indicated eicosanoids (HETEs) at the indicated concentrations for 1 h. Cell lysates were analyzed by immunoblotting for ABCG1 or β-actin. Blot is representative of three different experiments. E, C57BL6/J bone marrow-derived macrophages were treated with vehicle control (CTR) or arachidonic acid (AA) at the indicated concentrations for 16 h. Cell lysates were analyzed by immunoblotting for ABCG1 or β-actin. Blot is representative of three different experiments. F, C57BL6/J bone marrow-derived macrophages were pre-treated with 10 μm lactacystin, 10 μm MG132, or vehicle control DMSO for 30 min prior to the addition of 500 nm 12SHETE or vehicle control ethanol for an additional 30 min. Cell lysates were analyzed by immunoblotting for ABCG1 or β-actin. Blot is representative of five different experiments. G, ABCG1 was isolated by immunoprecipitation, and blots were probed with phosphoserine or ABCG1 antibodies. Blot is representative of three different experiments.