FIGURE 5.

12/15LO regulates activation of p38 and JNK MAPK pathways. Cell lysates were analyzed by immunoblotting using antibodies specific for the phosphorylated forms of p38, JNK, or MKK3/6. Bands were normalized to total p38, total JNK, or β-actin. A, C57BL6/J bone marrow-derived macrophages were incubated with 500 nm 12SHETE for 1–60 min. Cell lysates were analyzed for MAPK pathway activation by immunoblot analysis. Blot is representative of three different experiments. B, cell lysates from B6 or LOTG bone marrow-derived macrophages were analyzed for MAPK pathway activation by immunoblot analysis. Blot is representative of four different experiments. C, cell lysates from B6 or LOKO bone marrow-macrophages were analyzed for MAPK pathway activation by immunoblot analysis. Blot is representative of four different experiments.