Skip to main content
. 2009 Aug 27;284(45):31303–31314. doi: 10.1074/jbc.M109.028910

FIGURE 8.

FIGURE 8.

The MAPK pathway regulates 12/15LO-mediated down-regulation of ABCG1. A, JNK1-deficient (JNK1 KO), JNK2-deficient (JNK2 KO), MKK3-deficient (MKK3KO), or C57BL6/J (B6) control bone marrow-derived macrophages were incubated with recombinant adenovirus expressing either 12/15LO (+AdLO) or control LacZ (+AdLacZ) for 48 h. Cell lysates were analyzed by immunoblotting for ABCG1 or β-actin. Blot is representative of four different experiments. B, JNK1-deficient (JNK1 KO), JNK2-deficient (JNK2 KO), MKK3-deficient (MKK3 KO), or C57BL6/J (B6) control macrophages were treated with 500 nm 12SHETE or vehicle control ethanol for 30 min. Cell lysates were analyzed by immunoblotting for ABCG1 or β-actin. Blot is representative of four different experiments. C, ABCG1 was isolated by immunoprecipitation, and blots were probed with phosphoserine or ABCG1 antibodies. Blot is representative of three different experiments.