FIGURE 6.

ITC analysis of schistosome eIF4E cap binding and conformational differences between schistosome and human eIF4E. A, m⁷GpppG titration. B, m^2,2,7GpppG titration. The top panels show the raw data, and the bottom panels show the integrated data with the continuous lines representing the fit of the data to a single site binding model. The insets in the upper panels represent buffer injection alone. Similar values were obtained with m⁷GTP and m^2,2,7GTP. C, structural comparison of human (blue) and schistosome (yellow) eIF4Es around H2-S4 loop.