Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 15;284(45):31401–31411. doi: 10.1074/jbc.M109.033381

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Localization of MAA [CI-α2 (22 kDa)) in the eye. A, Western blot analysis using polyclonal antibody against bovine MAA (CI-α2 (22 kDa)) to detect MAA in different rat ocular tissues. Samples were analyzed on 14% SDS-PAGE. Total protein (40 μg) from cornea (lane 1), retina (lane 2), iris and CB (lane 3), and RPE and choroid (lane 4) were used. Purified bovine MAA (CI-α2 (22 kDa)) loaded (5 μg) in lane 5 was used as the positive control. B and C, MAA (CI-α2 (22 kDa)) in the rat eye (frozen sections). Naive rat eye was examined immunohistochemically for MAA using polyclonal antibody against bovine MAA. B, MAA is constitutively expressed on the iris (I) and ciliary body (CB); MAA was not detected on the cornea (Co). No staining was observed in the control section stained with normal rabbit serum (C). The data shown are representative of three separate experiments. AC, anterior chamber. Objective magnification was ×10.