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ATPase activity of the ClpA Walker B variants in the absence and presence of various substrates. The ATPase activity of 0.1 μm ClpA was measured in presence of 0.05 μm ClpP. 3 μm GFP-ssrA (a), 3 μm FRli-GFP (plus 0.6 μm ClpS) (b), 6 μm FRli-T4 lysozyme (plus 0.6 μm ClpS) (c), or 6 μm His6-TEV-FimAta-li-ssrA (d) was added where indicated (+). The error bars represent the standard deviation of at least three independent measurements. WT, wild type.
