FIGURE 7.

Soluble FB enhanced cytokine production of WT, but not integrin αIIb-deficient, BMMCs in response to S. aureus with FB-binding capacity. A, WT or integrin αIIb-deficient BMMCs were incubated with heat-killed S. aureus labeled by Cell Tracker Orange in the presence or absence of 500 μg/ml soluble FB for 2 h. WT, but not integrin αIIb-deficient, BMMCs were covered with SA aggregates in the presence of soluble FB (arrowhead). B, WT or integrin αIIb-deficient BMMCs were incubated with 100 ng/ml LPS, 100 μg/ml heat-killed E. coli, 100 μg/ml S. aureus, or PBS as control in the presence or absence of 500 μg/ml soluble FB for 8 h. The ratio of the amounts of IL-6 released in the presence of soluble FB to those of IL-6 in the absence of soluble FB was measured. Data are representative of three independent experiments. All data points correspond to the mean ± S.D. ** (p < 0.01) indicates statistical differences. C, WT or integrin αIIb-deficient BMMCs were incubated with the indicated concentrations of LPS in FB- or BSA-coated plates. Data represent three independent experiments. All data points correspond to the mean ± S.D. * (p < 0.05) indicate statistical differences.