Expression levels of some key autophagy genes in the livers of mice were suppressed by the HFD. mRNAs from the liver samples of mice described in Fig. 1 were extracted, converted into cDNA, and used for quantifications of target genes. Transcripts of ulk2 (A), beclin1 (B), vps34 (C), atg12 (D), and gabarapl1 (E) were measured by using real-time PCR and normalized to the level of 36B4 transcript. *, p < 0.05; **, p < 0.01; ND (n = 10) versus HFD (n = 11).