Table 2. Primer sequences used for quantitative RT-PCR.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Fragment size (bp)
GADPH	GCCTTCCGTGTTCCTACCC	CAGTGGGCCCTCAGATGC	117
TNF-α	CGCTCTTCTGTCTACTGAACTTCG	GATGATCTGAGTGTGAGGGTCTGG	115
TLR-4	CACTGTTCTTCTCCTGCCTGAC	AGGGACTTTGCTGAGTTTCTGATC	104

Primers were designed with the help of Beacon Designer software (Bio-Rad, Hercules, CA, USA) and provided by Invitrogen.