Table 2. Primer sequences used for quantitative RT-PCR.
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Fragment size (bp) |
GADPH | GCCTTCCGTGTTCCTACCC | CAGTGGGCCCTCAGATGC | 117 |
TNF-α | CGCTCTTCTGTCTACTGAACTTCG | GATGATCTGAGTGTGAGGGTCTGG | 115 |
TLR-4 | CACTGTTCTTCTCCTGCCTGAC | AGGGACTTTGCTGAGTTTCTGATC | 104 |
Primers were designed with the help of Beacon Designer software (Bio-Rad, Hercules, CA, USA) and provided by Invitrogen.