FIGURE 2.
Aldosterone stimulates edn1 mRNA and hnRNA in collecting duct cells. A, growth-arrested confluent monolayers of mpkCCDc14, OMCD1, or mIMCD-3 cells were treated with vehicle (open bars) or 1 μm aldosterone (closed bars) for 1 h. Steady-state edn1 mRNA was measured by QPCR, normalized to β-actin, and expressed as mRNA -fold change relative to vehicle ± S.E. B, aldosterone dose-response studies were conducted on mIMCD-3 cells treated with vehicle or aldosterone (0.01, 0.1, or 1 μm) for 1 h. Steady-state mRNA of edn1 or sgk1 was quantified as above. C, levels of edn1 hnRNA were also determined from dose-response studies conducted on mIMCD-3 cells. hnRNA was measured by SYBR Green QPCR, normalized to gapdh, and expressed as hnRNA -fold change relative to vehicle ± S.E. (n ≥ 3, *, p < 0.05, **, p < 0.005).