Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 4;284(44):30105–30112. doi: 10.1074/jbc.M109.044081

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — Western blot analysis of total soluble proteins isolated from wild-type SS1 and Rubisco small subunit mutants. Protein extracts (60 μg/lane) of cells grown at either 25 or 35 °C in darkness were fractionated by gradient gel (7.5–15% polyacrylamide) electrophoresis (43), blotted to nitrocellulose, probed with rabbit anti-Chlamydomonas Rubisco immunoglobulin G (0.5 μg/ml) (38), and detected by enhanced chemiluminescence (Amersham Biosciences) (44). The Rubisco 55-kDa large subunit (LS) and 16-kDa small subunit (SS) are indicated.