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. 2009 Sep 9;284(44):30159–30166. doi: 10.1074/jbc.M109.027995

FIGURE 1.

FIGURE 1.

PTEN catalytic activity and PDZ binding domain are not absolutely required for complex assembly. A, PC3 WT- and CS (inactive)-PTEN-inducible cells were induced with Dox and run through a gel filtration column. Fractions were collected and immunoblotted with anti-PTEN antibody. Higher molecular mass and monomeric fractions are depicted as II and I, respectively. B, PC3 WT- and CS-PTEN-inducible cells were treated with 2 μg/ml Dox for 36 h, and cell lysates were immunoblotted (IB) with antibodies against PTEN, V5 tag and the PTEN downstream target, P-AKT, and total AKT, using anti-actin as a loading control. C, PC3 cells were transfected with WT- or ΔPDZ-PTEN (PTEN lacking the PDZ binding domain) and run on a gel filtration column. Complex (19–21) and monomer (33–36) fractions were collected and immunoblotted with PTEN antibody. D, complex fractions (19–21) from PC3 WT-PTEN-induced (+Dox) and uninduced (−Dox) were immunoblotted with an antibody against hDLG.