FIGURE 2.

Point mutation of the tyrosine kinase domain results in impaired signaling ability. A, N620K point mutation, which is associated with recessive Robinow syndrome, results in impaired Wnt5a-mediated inhibitory activity. ∼40 h post-transfection with STF reporter and Ror2 variants, HEK293 cells were treated with vehicle, Wnt3a protein alone, Wnt5a protein alone, or Wnt3a protein plus Wnt5a protein for an additional 20 h. Following reporter assay, cell lysates were examined for receptor expression via Western blot analysis with α-Ror2 antibody. GFP, green fluorescent protein; WT, wild type. B, point mutation of tyrosine residues within the catalytic loop of the tyrosine kinase domain results in impaired inhibitory activity. Cells were transfected with Ror2 deletion variants described in text and treated as indicated above. Following reporter assay, cell lysates were examined for receptor expression via Western blot analysis with α-Ror2 antibody.