FIGURE 4.

ZBP-89 binds to the G-strings in two additional regulatory elements of the mouse Gata1 gene. A, schematic of the cis-acting regions of the mouse Gata1 gene containing G-strings and GATA sites. The sequences of the probes and competitors used for EMSA are indicated. B, binding of GST-ZBP-89 DBD to the G1HE-(201–235) (lanes 1–5), double GATA (lanes 6–10), and first intron (lanes 11–15) probes was examined by EMSA. One μg of recombinant protein was incubated with probe. Note that wild type (WT) competitor (lanes 2, 3, 7, 8, 12, and 13), but not the G₄ (MT) competitor (lanes 4, 5, 9, 10, 14, and 15), displaced probe binding of GST-ZBP-89 DBD.