FIGURE 7.

The G₅ string is required for ZBP-89-dependent activation of the G1HE-(124–235)-luc reporter. A, QT-6 cells were co-transfected with 20 ng of wild type or mutant G1HE-(124–235)-luc reporter, 5 ng of a Renilla luciferase control reporter, and 0, 100, or 250 ng of a ZBP-89 expression plasmid with (gray bars) or without (black bars) 100 ng of a GATA-1 expression plasmid. Wild type and mutant (GATA site mut, G₄ mut, and GATA site + G₄ mut) G1HE-(124–235) reporters were examined. B, QT-6 cells were co-transfected with 100 ng of an expression plasmid of GATA-1 (wild type or mutant), 250 ng of a ZBP-89 expression plasmid, 20 ng of G1HE-(124–235)-luc reporter, and 5 ng of a Renilla luciferase control reporter. The reporter activity of the expression vector with no insert was set to 1.0. FL, full-length; dNT, the N-terminal deleted GATA-1 mutant protein; dNF, the N-finger deleted GATA-1 mutant protein. *, p < 0.05 versus ZBP-89 without the GATA-1 expression plasmid.