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. 2009 Sep 3;284(44):30209–30217. doi: 10.1074/jbc.M109.024208

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — HNK-1 is expressed in the PSD fraction from mouse hippocampi. A, developmental expression of HNK-1 glyco-epitope in mouse hippocampi. Hippocampal P2 fractions (5 μg of proteins) obtained at the indicated developmental stages (E16, embryonic day 16; P7 to P210, postnatal day 7–210) were immunoblotted (IB) with HNK-1 monoclonal antibody. Note that the expression of HNK-1 completely disappeared at P14 in the GlcAT-P^−/− mice (right lane). B, biochemical fractionation of HNK-1 carrier proteins and PSD-95 in hippocampi. (S1, postnuclear supernatant; P2, crude synaptosome; P3, light membrane). Five micrograms of proteins obtained from different fractions were immunoblotted for HNK-1 and PSD-95.