FIGURE 2.

Immunoprecipitation analysis of the PSD fractions from mouse hippocampi. A, GluR1 and GluR2 were immunoprecipitated (IP) with GluR1- and GluR2-specific antibodies under SDS-denaturing conditions, respectively. The immunoprecipitates were treated with (+) or without (−) N-glycosidase F and then immunoblotted (IB) for HNK-1 (upper panel) and GluR1 or GluR2 (lower panel). B, NR1 and NR2 were immunoprecipitated and immunoblotted with HNK-1 antibody (upper panel), NR1 (middle panel), and NR2 (lower panel).