FIGURE 3.
Subcellular localization of endogenous Bfl-1 in human PBLs and tumoral cell lines. a, activated PBLs were treated with 0 μm (0) or 2 μm etoposide (Eto) for 12 h to induce apoptosis. Cytosolic (C) and mitochondrial (M) protein extracts were prepared and protein was assessed by Western blot using anti-Bfl-1, anti-Bcl-xL, and anti-Bax antibodies. Mn-SOD is a marker of mitochondrial fractions. Data are representative of three independent experiments. b, BP3 and IM9 cells were harvested, and cytosolic and mitochondrial protein extracts were prepared, and proteins were assessed by Western blot as in a. Data are representative of three independent experiments.