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. 2009 Sep 15;284(44):30257–30263. doi: 10.1074/jbc.M109.040824

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Importance of helix α9 in the mitochondrial localization of Bfl-1. FL-Bfl-1 or Δα9-Bfl-1 was translated in vitro in the presence of radioactive methionine and then incubated with purified liver rat mitochondrial. After separation by centrifugation, supernatant and mitochondrial pellets were analyzed by SDS-PAGE and quantified using a PhosphorImager. Localization of FL-Bfl-1 was assessed with or without alkali treatment (AT) before separation. Results are expressed as mean ± S.E. of three independent experiments.