FIGURE 1.

MLCL-adriamycin-agarose affinity chromatography profile and Western blot analysis. A, 4 ml of partially purified MLCL-AT was loaded onto 1 ml of adriamycin-agarose affinity gel, which was pre-equilibrated with 0.75 mg of MLCL. The gel was washed with 12 column volumes of 0.1 m borate buffer, pH 8.0, until no more protein was eluted. Finally, MLCL-AT was eluted (arrow) by the addition of 1 mm MLCL or 1 mm linoleoyl coenzyme A. B, Western blot of fraction 10 eluted with MLCL using the polyclonal antibody to MLCL AT and Western blot of fraction 10 eluted with linoleoyl coenzyme A. Origin and molecular mass are indicated on the right. Representative blots are depicted.