FIGURE 3.

Knockdown of ACSL3 suppresses the activity of lipogenic transcription factors in rat primary hepatocytes. ACSL3-a or ACSL3-b siRNA were transfected in rat primary hepatocytes, and reporter gene activities were quantified after 50 h. A and B, for SREBP, firefly luciferase reporter of SRE sequence on FAS gene were transfected for reporter gene assay. Cells in B were also transfected with pCMV-SREBP1-c, a constitutively active form of SREBP. C and D, firefly luciferase reporter driven by the ACC carbohydrate response element-containing promoter region was transfected for measurement of ChREBP in cells treated with 5 mm (C) and 25 mm glucose (D). E and F, pSG5-GAL4-hPPAR-γ expression plasmids were co-transfected with TK-MH-UAS-Luc reporter plasmids in cells treated with DMSO (E) or 10 μm rosiglitazone (F) for 18 h to determine whether synthetic ligands normalize PPAR-γ. G and H, pCMX-hLXR-α expression plasmid and TK-hcyp7a-LXRE(X3)-Luc reporter plasmid were used for measurement of LXR-α activity in cells treated with DMSO (G) or 15 μm T0901317 (H) for 18 h. Values shown are mean ± S.E. from a representative experiment performed in triplicate that was repeated two or three times. *, p < 0.05 when compared with controls.