Skip to main content
. 2009 Sep 9;284(44):30565–30573. doi: 10.1074/jbc.M109.059444

FIGURE 1.

FIGURE 1.

Mre11 is required for overall NHEJ repair. A, diagram of construction of NHEJ substrate pPHW1 (adapted from ref. 32). An ORF is dominant over the downstream gpt ORF. Reconstituted translation of the gpt ORF will be resistant to XHATM selection. Both precise and error-prone rejoining as well as deletion during NHEJ can be detected. The total NHEJ efficiency is obtained from the formation efficiency of XHATM-resistant colonies. The repair events, i.e. D-NHEJ or C-NHEJ, are then assessed by analysis of the end junction sequence. B, Mre11 protein knockdown with siRNA. Levels of Mre11 protein and I-SceI expression were determined by Western blotting. β-Actin was included as a loading control. C, overall I-SceI-induced DSB end-rejoining efficiency in HEK293/pPHW1 cells with or without Mre11 knockdown was measured by cells resistant to XHATM selection. *, statistical difference in repair efficiency between control siRNA versus Mre11 siRNA (p < 0.05).