Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 9;284(44):30565–30573. doi: 10.1074/jbc.M109.059444

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Rescue of suppressed D-NHEJ by expression of siRNA-resistant wild-type Mre11. A, quantitative real-time PCR to determine the effect of Mre11 knockdown on end joining. The arrows indicate the I-SceI cleavage sites, and the bold arrow indicates the precise religation product as well as the probe C complementary sequence. B, Mre11 knockdown inhibits D-NHEJ. C, Mre11 knockdown does not affect C-NHEJ. **, statistical difference in D-NHEJ repair efficiency between control siRNA versus Mre11 siRNA (p < 0.001). D, wild-type Mre11 rescues D-NHEJ after endogenous Mre11 is knocked down in HEK293/pPHW1 cells. **, statistical difference between control cells and wild-type Mre11-rescued cells (p < 0.001). E, wild-type Mre11 has no effects on C-NHEJ after endogenous Mre11 is knocked down in HEK293/pPHW1 cells. F, protein expression of endogenous Mre11 and exogenous Myc-Mre11 after rescue by transfection of siRNA-resistant Myc-tagged wild-type Mre11. β-Actin was included as a loading control.

FIGURE 4. — Rescue of suppressed D-NHEJ by expression of siRNA-resistant wild-type Mre11. A, quantitative real-time PCR to determine the effect of Mre11 knockdown on end joining. The arrows indicate the I-SceI cleavage sites, and the bold arrow indicates the precise religation product as well as the probe C complementary sequence. B, Mre11 knockdown inhibits D-NHEJ. C, Mre11 knockdown does not affect C-NHEJ. **, statistical difference in D-NHEJ repair efficiency between control siRNA versus Mre11 siRNA (p < 0.001). D, wild-type Mre11 rescues D-NHEJ after endogenous Mre11 is knocked down in HEK293/pPHW1 cells. **, statistical difference between control cells and wild-type Mre11-rescued cells (p < 0.001). E, wild-type Mre11 has no effects on C-NHEJ after endogenous Mre11 is knocked down in HEK293/pPHW1 cells. F, protein expression of endogenous Mre11 and exogenous Myc-Mre11 after rescue by transfection of siRNA-resistant Myc-tagged wild-type Mre11. β-Actin was included as a loading control.