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. 2009 Aug 31;284(44):30583–30593. doi: 10.1074/jbc.M109.010678

FIGURE 5.

FIGURE 5.

Role of ERK and JNK in FGF2 activation of the Gdnf promoter. Primary rat astrocytes were co-transfected with 50 ng of Elk-1 trans-activator plasmid (pFA2/Gal4-Elk-1), 50 ng of pRL-null vector, and 0.5 μg of the reporter plasmid pFR-Luc (A), the Egr-1 promoter-reporter plasmid pEgr1-Luc(−780/+1) (B), or the Gdnf promoter-reporter plasmid pGDNF Luc(−493/+3) (C), together with or without 0.2 μg of a plasmid expressing DN-MEK1 (pCGN1/MEK DN), DN-ERK2 (pHA-ERK2 K52R), or DN-JNK1 (pSRα/HA-JNK T183A/Y185F), as indicated. Twenty-four hours after transfection, the cells were treated with 0 or 10 ng/ml of FGF2 for 8 h. The resulting firefly luciferase activity was normalized to the Renilla luciferase activity. The data represent the mean ± S.D. (error bars) of three independent experiments performed in triplicate. *, p < 0.05; **, p < 0.01, compared with FGF2-treated control cells transfected with empty vector (Student's t test).