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. 2009 Sep 4;284(44):30643–30651. doi: 10.1074/jbc.M109.044099

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Gel mobility shift assay of the non-covalent complexes between MbtRNA^Ser and various mMbSerRS mutants. MbtRNA^Ser was incubated with different mMbSerRS variants (final concentration of tRNA and enzymes was 1.1 and 0.6 μm, respectively) and subjected to PAGE under native conditions: WT, lane b; R38A, lane c; R76A, lane d; R78A, lane e; K79A, lane f; K87A, lane g; K88A, lane h; Y89A, lane i; K90A, lane j; R94A, lane k; K141A, lane l; N142A, lane m; and R143A, lane n. Non-complexed mMbSerRS (8.3 pmol) and MbtRNA^Ser (14.8 pmol) were loaded on the gel as electrophoretic mobility markers (lanes a and o, respectively). Non-covalent complexes and non-complexed tRNA are marked with black and white arrows, respectively.