TABLE 1.
SerRS variant | ka1 | kd1 | ka2 | kd2 | KD | Relative KDa | χ2b |
---|---|---|---|---|---|---|---|
m−1s−1/105 | s−1/10−1 | s−1/10−2 | s−1/10−3 | m/10−7 | |||
WT | 1.471 ± 0.010 | 1.239 ± 0.009 | 1.482 ± 0.006 | 2.513 ± 0.003 | 1.22 | 1 | 2.71 |
R38A | 0.442 ± 0.001 | 0.342 ± 0.001 | 0.832 ± 0.004 | 9.949 ± 0.017 | 4.22 | 3.5 | 0.20 |
R78A | 0.558 ± 0.002 | 0.417 ± 0.002 | 1.204 ± 0.004 | 4.192 ± 0.006 | 1.93 | 1.6 | 0.28 |
K79A | 0.299 ± 0.001 | 0.231 ± 0.001 | 0.283 ± 0.002 | 7.543 ± 0.029 | 5.61 | 4.6 | 0.18 |
K87A | 0.983 ± 0.013 | 0.714 ± 0.011 | 1.345 ± 0.014 | 3.552 ± 0.011 | 1.52 | 1.2 | 1.95 |
K88A | 1.175 ± 0.004 | 0.862 ± 0.003 | 0.993 ± 0.002 | 2.703 ± 0.002 | 1.57 | 1.3 | 0.53 |
Y89A | 0.568 ± 0.002 | 0.498 ± 0.003 | 1.048 ± 0.004 | 5.494 ± 0.008 | 3.01 | 2.5 | 0.20 |
K90A | 0.572 ± 0.005 | 0.547 ± 0.006 | 1.606 ± 0.005 | 4.568 ± 0.006 | 2.12 | 1.7 | 0.77 |
R94A | 58.10 ± 1.70 | 47.6 | 0.07 | ||||
K141A | 0.264 ± 0.001 | 0.237 ± 0.001 | 0.515 ± 0.003 | 6.169 ± 0.013 | 4.89 | 4.0 | 1.34 |
N142A | 1.423 ± 0.005 | 1.212 ± 0.004 | 0.673 ± 0.002 | 5.578 ± 0.005 | 3.86 | 3.2 | 0.77 |
R143A | 0.324 ± 0.001 | 0.444 ± 0.002 | 0.605 ± 0.004 | 11.27 ± 0.03 | 8.90 | 7.3 | 0.09 |
Relative KD is determined as KD(mutant)/KD(WT).
Statistical value describing the closeness of fit. Values <10 are acceptable (34).