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Stoichiometry of BppU·BppL·DARPin complexes
| Proteins | Theoreticalmolecular weight | Molecular weight measuredby MALS/refractometry/SEC | Stoichiometry(BppU3-BppL9):DARPins | (BppU3-BppL9)/DARPin R1h QELS |
|---|---|---|---|---|
| Da | Da | nm | ||
| (BppU3-BppL9) | 263,640 (9 × 17,970 + 3 × 33,970) | 255,000 | 6.0 | |
| (BppU3-BppL9) + DARPin 18 | 308,025 (263,640 + 3 × 14,795) | 291,000 | 1:3 | 6.6 |
| (BppU3-BppL9) + DARPin 19 | 307,971 (263,640 + 3 × 14,777) | 289,000 | 1:3 | 6.4 |
| (BppU3-BppL9) + DARPin 20 | 307,788 (263,640 + 3 × 14,716) | 285,000 | 1:3 | 6.4 |
