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Stoichiometry of BppL·DARPin complexes
| Proteins | Theoreticalmolecular weight | Molecular weightmeasured by MALS/refractometry/SEC | StoichiometryBppL3:DARPins |
|---|---|---|---|
| Da | Da | ||
| BppL3 | 53,910 (3 × 17 970) | 54,000 | |
| BppL3 + DARPin 18 | 53,910 + 14,795 | 63,627 | 1:1 |
| BppL3 + DARPin 19 | 53,910 + 14,777 | 60,370 | 1:1 |
| BppL3 + DARPin 20 | 53,910 + 14,716 | 61,618 | 1:1 |
